12936 Adam6b Status: ES cell colonies screened / QC no positives
| Gene | Adam6b |
| Chromosome | 12 |
| Deletion Start | 113,489,568 |
| Deletion End | 113,491,925 |
| Deletion Size | 2,358 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,490,414 | 113,490,439 | GGGTCGGCTTATAATTACTATGTGTC |
| TUP | 113,490,453 | 113,490,471 | AAATTTAAGCCTGATGCAG |
| TUR | 113,490,498 | 113,490,522 | CAGCTGGATTAACCTGGTTATCTTG |
| TDF | 113,491,557 | 113,491,580 | TGCCATTGTCATTTTGGTTGGAAG |
| TDP | 113,491,581 | 113,491,610 | CCTCCAGAGTGCAAAGAAGAGGGACTAAGT |
| TDR | 113,491,613 | 113,491,632 | GGGACCCACTGTCTATGCTC |
PCR Primers
| Primer | Sequence |
| SU | TCAGAGACACTCCTTGCTGC |
| SD | ATCCCATCAGTCTCGTGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
