12939   Gpbar1     Status: ES cell colonies screened / QC positives

GeneGpbar1
Chromosome1
Deletion Start74,278,603
Deletion End74,279,692
Deletion Size1,090
Genome Build38
MGI IDMGI:2653863
NCBI Gene ID227289
BAC ID65N10
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 1

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,278,99174,279,011GGGTCAGCTCCCTGTTCTTTG
TUP74,279,01674,279,037CCTGCCTGCTCTGGGCTGGAAC
TUR74,279,03874,279,059AGTTGGCATCAGGGCTCCAATG
TDF74,279,47674,279,494GCTGCCCAAAGGTGTCTAC
TDP74,279,50174,279,527TTCGAGGAAGAGCCAAGAGGGACAATC
TDR74,279,54874,279,566TGCATTGGCTACTGGTGTG

PCR Primers

PrimerSequence
SUGTGGGAACTGTGGTTATCGC
SDTTGACTTTGATTCGCTGGTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR69 bp++-
TDFTDR91 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12939A-C6VGB6C57BL/6NTac
12939A-D8VGB6C57BL/6NTac
12939A-F12VGB6C57BL/6NTac
12939A-H7VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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