12947 Ranbp6 Status: ES cell colonies screened / QC no positives
| Gene | Ranbp6 |
| Chromosome | 19 |
| Deletion Start | 29,812,947 |
| Deletion End | 29,809,598 |
| Deletion Size | 3,350 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,812,749 | 29,812,772 | GAAGAGCAGGTTATGAGGTGAGAC |
| TUP | 29,812,724 | 29,812,746 | ATGGCTGCCGCACTGCTACGACG |
| TUR | 29,812,659 | 29,812,679 | TGACATCCCTCTGCACTTCAG |
| TDF | 29,810,224 | 29,810,244 | TGCAGTCCAACCTCATTCAAG |
| TDP | 29,810,196 | 29,810,219 | TTGAGTATTTCCGGTGGCCGATGC |
| TDR | 29,810,160 | 29,810,181 | GTCTGACTTCAGGGTTGTTGTC |
PCR Primers
| Primer | Sequence |
| SU | TCTCCTTCCAGCCTCTACAG |
| SD | GGAAGAACAAGTCTGCCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
