| Gene | Syna |
| Chromosome | 5 |
| Deletion Start | 134,560,090 |
| Deletion End | 134,558,176 |
| Deletion Size | 1,915 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,559,889 | 134,559,912 | CACTAAGGACTTGGACAGAGACAC |
| TUP | 134,559,854 | 134,559,880 | CTTCGAATCATGTACTCAGCCCGGACC |
| TUR | 134,559,834 | 134,559,853 | ATAGGGTATGGGCCGGAGAG |
| TDF | 134,558,445 | 134,558,462 | GCTGCCCTCTTGGTTGAC |
| TDP | 134,558,416 | 134,558,443 | TCCCTCATGGCTCCCATTCTCTTTATCC |
| TDR | 134,558,353 | 134,558,373 | TTCGCCGCGATACAGAATGAG |
PCR Primers
| Primer | Sequence |
| SU | ACATCCTCCACTCTCATGCC |
| SD | TCCGCTAACAGACTGGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12948A-C6 | VGB6 | C57BL/6NTac | |
| 12948A-E8 | VGB6 | C57BL/6NTac | |
| 12948A-G7 | VGB6 | C57BL/6NTac | |
| 12948A-G8 | VGB6 | C57BL/6NTac | |
