12954 Gm443 Status: ES cell colonies screened / QC positives
| Gene | Gm443 |
| Chromosome | 5 |
| Deletion Start | 25,022,110 |
| Deletion End | 25,023,809 |
| Deletion Size | 1,700 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TGGCACGGTCTTTGTTGTTC |
| TUP | | | ACTGACAGAAGCCTTGCTGGAGCAG |
| TUR | | | CGGAGCTGGGTTGGAGTTAG |
| TDF | | | TCGGTGGACCAGGCTAAC |
| TDP | | | TGTGGCATCATGGTGATTCAAGCCA |
| TDR | | | TGCCCAGCTTCTCACTCAG |
PCR Primers
| Primer | Sequence |
| SU | TGACTTCATTCCCTATCTCTG |
| SD | TCATCTTTGTTGTTCTGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12954B-C5 | VGB6 | C57BL/6NTac | |
