12959 4932411N23Rik Status: ES cell colonies screened / QC no positives
| Gene | 4932411N23Rik |
| Chromosome | X |
| Deletion Start | 126,815,477 |
| Deletion End | 126,814,047 |
| Deletion Size | 1,431 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,815,299 | 126,815,322 | CCCACTACAAGAAATGGAGCAAAG |
| TUP | 126,815,273 | 126,815,297 | TTTGGTTTGGTCACAACCACAGCCA |
| TUR | 126,815,253 | 126,815,272 | CTCTTCCTCAGCCACCTCTG |
| TDF | 126,814,593 | 126,814,613 | GTGGGTACTCGCTCAGCAAAG |
| TDP | 126,814,560 | 126,814,589 | TGTCGTGCTAAATGGCTCAACTACTTGAAC |
| TDR | 126,814,533 | 126,814,557 | CCACTCAATACCACCAGTCTGTTTC |
PCR Primers
| Primer | Sequence |
| SU | AGTTCTCTGAAGCTTCCATGC |
| SD | TTATGGCAGCAGAGCCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
