12960 Noto Status: Cancelled
| Gene | Noto |
| Chromosome | 6 |
| Deletion Start | 85,423,994 |
| Deletion End | 85,428,129 |
| Deletion Size | 4,136 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,424,340 | 85,424,359 | GCCTCAGGCTCACAGGTATC |
| TUP | 85,424,362 | 85,424,387 | TATGGAAGCGGTGGCAGATAGCATCA |
| TUR | 85,424,404 | 85,424,421 | CCATCACCCTGGGCTAAC |
| TDF | 85,428,010 | 85,428,031 | GAAATTGCCTTCCTCCTCTGTC |
| TDP | 85,428,036 | 85,428,057 | AGGAGCCCTCCAGCAGCTCAGA |
| TDR | 85,428,072 | 85,428,091 | TCCCAACTCAGCATCTTCAC |
PCR Primers
| Primer | Sequence |
| SU | AAAGCACTGGAGTGGAGGAG |
| SD | GATGAATCCATCTTCAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
