12964 B020018G12Rik Status: ES cell colonies screened / QC no positives
| Gene | B020018G12Rik |
| Chromosome | 1 |
| Deletion Start | 175,978,906 |
| Deletion End | 175,978,353 |
| Deletion Size | 554 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 175,978,691 | 175,978,709 | GCGCTCTGTTGAAGGTTGG |
| TUP | 175,978,662 | 175,978,688 | TCTCTTTGTCACTGGAGTCCACGAAGA |
| TUR | 175,978,635 | 175,978,658 | TTGTCATGGATGTCTTCTTCAGTG |
| TDF | 175,978,419 | 175,978,439 | CAAGAGGAGAGGAGGCCGAAG |
| TDP | 175,978,396 | 175,978,417 | CGAAGCAGGAGTCCAGACCGGA |
| TDR | 175,978,362 | 175,978,386 | CCACCCAGTCAACAGAGGATTTATC |
PCR Primers
| Primer | Sequence |
| SU | AGACACAGGCTTTTCTTTAATC |
| SD | ATGTTTGTCTACAGCAATACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
