| Gene | Zglp1 |
| Chromosome | 9 |
| Deletion Start | 21,067,076 |
| Deletion End | 21,062,573 |
| Deletion Size | 4,504 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,066,958 | 21,066,977 | GAGCCTGGAGCCCTGAGATG |
| TUP | 21,066,931 | 21,066,956 | CTCACTCCCAATATCAGGTGCGTGGC |
| TUR | 21,066,900 | 21,066,919 | TTAGCTGGGATGGGCGATGG |
| TDF | 21,063,004 | 21,063,026 | CTGGAATTGAACTCTGGCCTTTG |
| TDP | 21,062,982 | 21,063,002 | CTTCTGACCCTCCAGGGCCCA |
| TDR | 21,062,948 | 21,062,969 | GGAGTCCTCTGGGTTCTACAAG |
PCR Primers
| Primer | Sequence |
| SU | TGTGATCGAAGATAGCTAGC |
| SD | TGCCGCCATTGTGTTGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12972A-B9 | VGB6 | C57BL/6NTac | |
| 12972A-F12 | VGB6 | C57BL/6NTac | |
| 12972A-E2 | VGB6 | C57BL/6NTac | |
| 12972A-H2 | VGB6 | C57BL/6NTac | |
