12976 Adra2c Status: ES cell colonies screened / QC positives
| Gene | Adra2c |
| Chromosome | 5 |
| Deletion Start | 35,279,889 |
| Deletion End | 35,281,339 |
| Deletion Size | 1,451 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,280,241 | 35,280,258 | TCGGGCAAGTGTGGTGTG |
| TUP | 35,280,260 | 35,280,282 | TGTATACCTGGCACTGGACGTGC |
| TUR | 35,280,284 | 35,280,303 | CGATGGACGAGGTGCAGAAG |
| TDF | 35,281,172 | 35,281,193 | CCCGGTCATCTACACTGTCTTC |
| TDP | 35,281,195 | 35,281,222 | ATCAGGACTTCAGGCGCTCTTTCAAGCA |
| TDR | 35,281,257 | 35,281,275 | GGAGGCCAGAGGGTCATTG |
PCR Primers
| Primer | Sequence |
| SU | CGTTGGGAAGCAGAGTGTC |
| SD | TGGCTGTCATTGTATTTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
