| Gene | Akp3 |
| Chromosome | 1 |
| Deletion Start | 87,125,011 |
| Deletion End | 87,127,968 |
| Deletion Size | 2,958 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,125,725 | 87,125,743 | AGCCGCGAGATTCGACCAG |
| TUP | 87,100,720 | 87,100,746 | TGCAACACCACATTTGGCAATGAGGTC |
| TUR | 87,100,695 | 87,100,713 | TCTTGGCACGGTACATCAC |
| TDF | 87,127,357 | 87,127,376 | GCAAGCCCTACACCTCCATC |
| TDP | 87,127,378 | 87,127,400 | TGTATGGCAACGGCCCAGGCTAT |
| TDR | 87,127,435 | 87,127,455 | CACCGCACTCACTGCTTTCAG |
PCR Primers
| Primer | Sequence |
| SU | GTTCTTCCAGGCCTACCTCC |
| SD | ATGGATTCAAGCTCGGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 25049 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12977A-F12 | VGB6 | C57BL/6NTac | |
| 12977A-G4 | VGB6 | C57BL/6NTac | |
