| Gene | Amh |
| Chromosome | 10 |
| Deletion Start | 80,805,251 |
| Deletion End | 80,807,732 |
| Deletion Size | 2,482 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,805,987 | 80,806,007 | GGCCTCAGAGACTCAGTCAAC |
| TUP | 80,806,012 | 80,806,037 | ATTTGCCCACAGTGATATGGGAGCCC |
| TUR | 80,806,047 | 80,806,068 | GTGGAGGCTCTTGGAACTTCAG |
| TDF | 80,807,392 | 80,807,410 | CAGTGCTCATCCCGGAGAC |
| TDP | 80,807,413 | 80,807,435 | ACCAAGCCAACAACTGCCAAGGC |
| TDR | 80,807,455 | 80,807,473 | TAGCGCGGATTACGGTCAG |
PCR Primers
| Primer | Sequence |
| SU | TATTTGTCCTGCCTTGAGCC |
| SD | CAGGCTGACTTGAAGCATTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12978A-B12 | VGB6 | C57BL/6NTac | |
| 12978A-E10 | VGB6 | C57BL/6NTac | |
| 12978A-F4 | VGB6 | C57BL/6NTac | |
| 12978A-H7 | VGB6 | C57BL/6NTac | |
