| Gene | Cd19 |
| Chromosome | 7 |
| Deletion Start | 126,414,833 |
| Deletion End | 126,409,311 |
| Deletion Size | 5,523 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,413,957 | 126,413,975 | CCGGTGGAATGCTTCAGAC |
| TUP | 126,413,932 | 126,413,955 | TCAGGGACCTGGACTGTGACCTAA |
| TUR | 126,413,912 | 126,413,930 | GCTCCCAGAGGACCTGTTC |
| TDF | 126,409,692 | 126,409,716 | CAGCAGAATTTCTGGGTCTCTAATC |
| TDP | 126,409,666 | 126,409,690 | TCCATCCCATGCCTAAACAGGGTCC |
| TDR | 126,409,617 | 126,409,636 | GGAGCTGAGGAGCTGCATAG |
PCR Primers
| Primer | Sequence |
| SU | CAGTAGTGAAGATGTTAGCC |
| SD | CCGGCTTCAGAGAATTTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12983A-A1 | VGB6 | C57BL/6NTac | |
| 12983A-D8 | VGB6 | C57BL/6NTac | |
