12985 Chrm2 Status: ES cell colonies screened / QC no positives
| Gene | Chrm2 |
| Chromosome | 6 |
| Deletion Start | 36,473,213 |
| Deletion End | 36,474,632 |
| Deletion Size | 1,420 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,473,648 | 36,473,668 | GCGTGGGTTCTTTCCTTCATC |
| TUP | 36,473,677 | 36,473,702 | CCCAGCCATTCTCTTCTGGCAGTTCA |
| TUR | 36,473,711 | 36,473,731 | CCCGTCTTCCACAGTCCTTAC |
| TDF | 36,474,412 | 36,474,433 | GCCATACAATGTCATGGTGCTC |
| TDP | 36,474,435 | 36,474,461 | TCAATACCTTCTGTGCACCCTGCATCC |
| TDR | 36,474,526 | 36,474,547 | GGTGGCGTTACAAAGTGCATAG |
PCR Primers
| Primer | Sequence |
| SU | TGGTGATATAAGCAAGCAGGC |
| SD | GTGTTAGCATGTAATGCGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 136 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
