12985 Chrm2 Status: ES cell colonies screened / QC no positives
Gene | Chrm2 |
Chromosome | 6 |
Deletion Start | 36,523,213 |
Deletion End | 36,524,632 |
Deletion Size | 1,420 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 36,523,648 | 36,523,668 | GCGTGGGTTCTTTCCTTCATC |
TUP | 36,523,677 | 36,523,702 | CCCAGCCATTCTCTTCTGGCAGTTCA |
TUR | 36,523,711 | 36,523,731 | CCCGTCTTCCACAGTCCTTAC |
TDF | 36,524,412 | 36,524,433 | GCCATACAATGTCATGGTGCTC |
TDP | 36,524,435 | 36,524,461 | TCAATACCTTCTGTGCACCCTGCATCC |
TDR | 36,524,526 | 36,524,547 | GGTGGCGTTACAAAGTGCATAG |
PCR Primers
Primer | Sequence |
SU | TGGTGATATAAGCAAGCAGGC |
SD | GTGTTAGCATGTAATGCGCC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 84 bp | + | + | - |
TDF | TDR | 136 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |