12988 Dhh Status: ES cell colonies screened / QC positives
| Gene | Dhh |
| Chromosome | 15 |
| Deletion Start | 98,728,734 |
| Deletion End | 98,724,369 |
| Deletion Size | 4,366 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GATGACAGAGGTAAGGAGAGTTTC |
| TUP | | | CCACCTGCTCTACCACACCACCA |
| TUR | | | TCATCCTCCAGGGTTGTTGTAG |
| TDF | | | CCGGATTCGACTGGGTCTAC |
| TDP | | | TACGAGTCCCGCAACCACATCCA |
| TDR | | | GACCATCATCTGGCCTCTACTG |
PCR Primers
| Primer | Sequence |
| SU | TGGTGTCCAGTTGTGACTGC |
| SD | TGTTAAAGCCAAGCTCCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
