| Gene | Gstp2 |
| Chromosome | 19 |
| Deletion Start | 4,041,936 |
| Deletion End | 4,040,290 |
| Deletion Size | 1,647 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,041,896 | 4,041,917 | ACTTCCCAAGTCCAGGTAGGAG |
| TUP | 4,041,835 | 4,041,859 | TTGCAGCCCACCATTCCTATCTGCC |
| TUR | 4,041,792 | 4,041,814 | CCTTGCAACCAGGAGGATTAATG |
| TDF | 4,040,612 | 4,040,628 | GTGGAGGTGGGGGTGAG |
| TDP | 4,035,697 | 4,035,715 | CCCCAGCCCCAGCCTCCTC |
| TDR | 4,040,550 | 4,040,568 | AGGAGGTGTGGGTCTCAGC |
PCR Primers
| Primer | Sequence |
| SU | TCTCTGACTATTCAGCACTTG |
| SD | GTGTGTGTGTGTATGAGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 126 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12998A-B9 | VGB6 | C57BL/6NTac | |
| 12998A-G11 | VGB6 | C57BL/6NTac | |
| 12998A-C7 | VGB6 | C57BL/6NTac | |
| 12998A-E8 | VGB6 | C57BL/6NTac | |
