| Gene | Htr1b |
| Chromosome | 9 |
| Deletion Start | 81,632,549 |
| Deletion End | 81,631,313 |
| Deletion Size | 1,237 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,632,467 | 81,632,488 | TCACCAACCTCTCCCACAACTG |
| TUP | 81,632,442 | 81,632,466 | CAGCGCCGACGGCTACATTTACCAG |
| TUR | 81,632,391 | 81,632,409 | GAGCGCCAACAAAGCAACC |
| TDF | 81,631,750 | 81,631,771 | CCTCGGTCACCTCCATTAACTC |
| TDP | 81,631,723 | 81,631,743 | TCCGGACGTGCCCAGTGAGTC |
| TDR | 81,631,679 | 81,631,700 | CTGAGACTCGCACTTTGACTTG |
PCR Primers
| Primer | Sequence |
| SU | GAGAGAAGCTGGACTGGGTG |
| SD | GCCAGGCTGTATGTAGAGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13007A-D2 | VGB6 | C57BL/6NTac | |
| 13007A-F5 | VGB6 | C57BL/6NTac | |
