13012 Ascl2 Status: ES cell colonies screened / QC positives
| Gene | Ascl2 |
| Chromosome | 7 |
| Deletion Start | 150,154,934 |
| Deletion End | 150,153,826 |
| Deletion Size | 1,109 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GCCCGTGAAGGTGCAAAC |
| TUP | | | ACTTCCCACCGCACCCGGTTCCT |
| TUR | | | CCCTGCTACGAGTTCTGGTG |
| TDF | | | GCGCCAACAAGAAGCTGAG |
| TDP | | | TAAGGTGGAGACGCTGCGCTCC |
| TDR | | | GCAGCGCACGAATGTACTC |
PCR Primers
| Primer | Sequence |
| SU | TGGTCACCGCAAAGCTTGG |
| SD | CTAGGACGCGATTTGCAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13012A-A3 | VGB6 | C57BL/6NTac | |
| 13012A-H2 | VGB6 | C57BL/6NTac | |
| 13012A-G10 | VGB6 | C57BL/6NTac | |
| 13012A-C4 | VGB6 | C57BL/6NTac | |
| 13012A-C5 | VGB6 | C57BL/6NTac | |
