13013 Myf5 Status: Cancelled
| Gene | Myf5 |
| Chromosome | 10 |
| Deletion Start | 107,485,928 |
| Deletion End | 107,484,013 |
| Deletion Size | 1,916 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,485,500 | 107,485,518 | GAGGGAACAGGTGGAGAAC |
| TUP | 107,485,474 | 107,485,497 | TTACAGCCTGCCGGGACAGAGCTG |
| TUR | 107,485,423 | 107,485,443 | AGGCTTACCATGCCGTCAGAG |
| TDF | 107,484,171 | 107,484,190 | TCCGTGTCCAGCTTGGATTG |
| TDP | 107,484,143 | 107,484,168 | TGTCCAGCATTGTGGATCGGATCACG |
| TDR | 107,484,122 | 107,484,141 | CAGCTCGGATGGCTCTGTAG |
PCR Primers
| Primer | Sequence |
| SU | TCATTAGCGTCAAAGGGACC |
| SD | CCTGTAATGGATTCCAAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
