| Gene | Myod1 |
| Chromosome | 7 |
| Deletion Start | 46,376,582 |
| Deletion End | 46,378,391 |
| Deletion Size | 1,810 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,377,053 | 46,377,071 | GGCCTTCGAGACGCTCAAG |
| TUP | 46,377,074 | 46,377,096 | CTGCACGTCCAGCAACCCGAACC |
| TUR | 46,377,131 | 46,377,152 | CAGACCTTCGATGTAGCGGATG |
| TDF | 46,378,008 | 46,378,029 | GCACACCTTCCTCTCCTTTCTC |
| TDP | 46,378,032 | 46,378,058 | TCCAGTCTAGCAAGTCCTCAGTTTCCC |
| TDR | 46,378,080 | 46,378,103 | CCTTCTCAAGTCTCCTGCCCATAG |
PCR Primers
| Primer | Sequence |
| SU | CAAGCTCCGCCCTACTACAC |
| SD | ACACCTGAGACCTGAGTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13015B-E5 | VGB6 | C57BL/6NTac | |
| 13015B-F4 | VGB6 | C57BL/6NTac | |
| 13015B-H12 | VGB6 | C57BL/6NTac | |
