| Gene | Ccl3 |
| Chromosome | 11 |
| Deletion Start | 83,649,274 |
| Deletion End | 83,648,170 |
| Deletion Size | 1,105 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,648,946 | 83,648,964 | GCTCTGCCTCTGGGATGTC |
| TUP | 83,648,917 | 83,648,943 | ATGGTGATGCTCTGGTGTCTTCTTTGC |
| TUR | 83,648,865 | 83,648,888 | TCTATGTGTTGCCCTGTGTCTTTC |
| TDF | 83,648,300 | 83,648,319 | GATCTGCGCTGACTCCAAAG |
| TDP | 83,648,267 | 83,648,294 | TGGGTCCAAGAATACATCACTGACCTGG |
| TDR | 83,648,231 | 83,648,248 | TTCCTCGCTGCCTCCAAG |
PCR Primers
| Primer | Sequence |
| SU | ATAGCCCTGGGTTGACACTG |
| SD | CCAGTCACTTGTTAAAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13024B-G1 | VGB6 | C57BL/6NTac | |
| 13024B-H5 | VGB6 | C57BL/6NTac | |
| 13024A-A10 | VGB6 | C57BL/6NTac | |
