| Gene | Zrsr1 |
| Chromosome | 11 |
| Deletion Start | 22,973,231 |
| Deletion End | 22,974,530 |
| Deletion Size | 1,300 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,973,355 | 22,973,376 | CAGAGGAGGACGATGATGTTTC |
| TUP | 22,973,377 | 22,973,405 | TGCTAACGAAGAACTTGCAGAGCGATTAC |
| TUR | 22,973,410 | 22,973,433 | TCATGTAATCTTTGCCGCTCTATC |
| TDF | 22,974,339 | 22,974,360 | ACCACTCTGGTTCATACCACTC |
| TDP | 22,974,362 | 22,974,386 | TCCAAGAGAAACAGGGAGTCCGAGA |
| TDR | 22,974,388 | 22,974,408 | TTCCACCTGTGAGGACTCTTC |
PCR Primers
| Primer | Sequence |
| SU | CAAACACTGGCCTTCGACTG |
| SD | AACAACATGGGAAGCCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13030A-E8 | VGB6 | C57BL/6NTac | |
| 13030A-E12 | VGB6 | C57BL/6NTac | |
