13034 Fasl Status: Cancelled
| Gene | Fasl |
| Chromosome | 1 |
| Deletion Start | 161,788,282 |
| Deletion End | 161,781,537 |
| Deletion Size | 6,746 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 161,788,005 | 161,788,022 | TCTGGTGGCTCTGGTTGG |
| TUP | 161,787,969 | 161,787,996 | TAGGAATGTATCAGCTCTTCCACCTGCA |
| TUR | 161,787,934 | 161,787,955 | CTAGGCTTACCTCACGGAGTTC |
| TDF | 161,781,928 | 161,781,948 | CCCTCTGGAATGGGAAGACAC |
| TDP | 161,781,899 | 161,781,924 | TGGAACCGCTCTGATCTCTGGAGTGA |
| TDR | 161,781,848 | 161,781,869 | ACACGAAGTACAACCCAGTTTC |
PCR Primers
| Primer | Sequence |
| SU | CTGCGGAAGAGCTAATGTCC |
| SD | TTCTCTGTGCCTCTGCATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
