| Gene | Mc1r |
| Chromosome | 8 |
| Deletion Start | 123,407,513 |
| Deletion End | 123,408,483 |
| Deletion Size | 971 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,407,581 | 123,407,599 | GGCCACCAACCAGTCAGAG |
| TUP | 123,407,602 | 123,407,627 | TTGGTGCCTGTATGTGTCCATCCCAG |
| TUR | 123,407,668 | 123,407,689 | GGCTATCACAACCAGCACATTC |
| TDF | 123,408,072 | 123,408,092 | TCTGCCTCGTCACTTTCTTTC |
| TDP | 123,408,096 | 123,408,119 | CCATGCTGGCACTCATGGCGATTC |
| TDR | 123,408,133 | 123,408,151 | CTGGCACGCTCTCGTGAAC |
PCR Primers
| Primer | Sequence |
| SU | GGCATGTCTCTACCTGAGCC |
| SD | CTTCATGTCTGTCCCAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13037A-B6 | VGB6 | C57BL/6NTac | |
| 13037A-B10 | VGB6 | C57BL/6NTac | |
| 13037A-D1 | VGB6 | C57BL/6NTac | |
