| Gene | Il12a |
| Chromosome | 3 |
| Deletion Start | 68,691,554 |
| Deletion End | 68,698,075 |
| Deletion Size | 6,522 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 68,691,599 | 68,691,620 | TGATGAGAGGAGAAGGCTTGAG |
| TUP | 68,691,621 | 68,691,647 | AAAGAACAGACAGAAGCCCTGGACCCA |
| TUR | 68,691,656 | 68,691,673 | GCTGGCAGCCCTTTAGTC |
| TDF | 68,697,970 | 68,697,990 | AAGCTCTGCATCCTGCTTCAC |
| TDP | 68,697,992 | 68,698,012 | CCTTCAGCACCCGCGTCGTGA |
| TDR | 68,698,017 | 68,698,038 | CAGATAGCCCATCACCCTGTTG |
PCR Primers
| Primer | Sequence |
| SU | CAAGAGTGGCTACTCGCAG |
| SD | TCTTCTGCTAACACATTGAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13045A-A9 | VGB6 | C57BL/6NTac | |
| 13045A-C6 | VGB6 | C57BL/6NTac | |
| 13045A-E8 | VGB6 | C57BL/6NTac | |
| 13045A-F6 | VGB6 | C57BL/6NTac | |
