| Gene | Il12b |
| Chromosome | 11 |
| Deletion Start | 44,404,040 |
| Deletion End | 44,413,238 |
| Deletion Size | 9,199 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,404,154 | 44,404,173 | AGCGCCAGCACCTAAACTTG |
| TUP | 44,404,174 | 44,404,197 | ATCTCCAGGCAGCCTGCTGGGAAG |
| TUR | 44,404,203 | 44,404,224 | GGTTGCCTACTTCAGACTTCAC |
| TDF | 44,412,560 | 44,412,577 | TGCAAAGGCGGGAATGTC |
| TDP | 44,412,578 | 44,412,601 | TGCGTGCAAGCTCAGGATCGCTAT |
| TDR | 44,412,610 | 44,412,628 | TGCCCACTTGCTGCATGAG |
PCR Primers
| Primer | Sequence |
| SU | CTCCAGGTAGCCTCTAGTTC |
| SD | TTGCATAATAGGGCCTGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13046D-F1 | VGB6 | C57BL/6NTac | |
| 13046A-A3 | VGB6 | C57BL/6NTac | |
