13047 Itgax Status: ES cell colonies screened / QC no positives
| Gene | Itgax |
| Chromosome | 7 |
| Deletion Start | 128,129,623 |
| Deletion End | 128,150,289 |
| Deletion Size | 20,667 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 128,130,306 | 128,130,326 | TGGATGCAGAGAAGCTGACAC |
| TUP | 128,130,329 | 128,130,354 | TTTCACATGGACGGTGCTGAGTTCGG |
| TUR | 128,130,360 | 128,130,384 | CAGGAACTATCATACTGGAGCACAC |
| TDF | 128,150,117 | 128,150,141 | CAGTACAAGGAGATGTTGGAGGAAG |
| TDP | 128,150,146 | 128,150,171 | TGGACAGTTTGTCTCAGACGGGACCC |
| TDR | 128,150,207 | 128,150,225 | GCGGGTTCAAAGACGATGG |
PCR Primers
| Primer | Sequence |
| SU | CATATATCCATCCGGGCAAG |
| SD | AGGATAACATGGAAGCACGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
