| Gene | Id1 |
| Chromosome | 2 |
| Deletion Start | 152,736,344 |
| Deletion End | 152,737,094 |
| Deletion Size | 751 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 152,736,500 | 152,736,520 | CAGGTGAACGTCCTGCTCTAC |
| TUP | 152,736,523 | 152,736,545 | CATGAACGGCTGCTACTCACGCC |
| TUR | 152,736,572 | 152,736,592 | CTTGCTCACTTTGCGGTTCTG |
| TDF | 152,736,923 | 152,736,943 | TTGTCCTTTCCAGTGGGTCTC |
| TDP | 152,736,950 | 152,736,974 | TATCTCGCTCTGGTGTTCACAGGCG |
| TDR | 152,736,998 | 152,737,017 | CGCCTCAGCGACACAAGATG |
PCR Primers
| Primer | Sequence |
| SU | GACCGTGAGAAGCTGATTCC |
| SD | CCCAGATTCTCAACACCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13053A-A10 | VGB6 | C57BL/6NTac | |
| 13053A-F11 | VGB6 | C57BL/6NTac | |
| 13053A-C10 | VGB6 | C57BL/6NTac | |
