13061 H2-Ab1 Status: Cancelled
| Gene | H2-Ab1 |
| Chromosome | 17 |
| Deletion Start | 34,263,315 |
| Deletion End | 34,269,159 |
| Deletion Size | 5,845 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,264,102 | 34,264,124 | GGTGTCTTCAGTCTCGCAGTTAC |
| TUP | 34,264,126 | 34,264,151 | CTTAAGATGCCTGGAGTGACCGTGCA |
| TUR | 34,264,160 | 34,264,178 | CCAGGCAACCTCTGAGAAC |
| TDF | 34,268,784 | 34,268,802 | TGGCAGGAAGAGGCTGAAG |
| TDP | 34,268,805 | 34,268,830 | TCAGCAGAGGAGATGGATCCCTCCAA |
| TDR | 34,268,846 | 34,268,868 | CCTCCCAGGGAATATGGAGAATC |
PCR Primers
| Primer | Sequence |
| SU | TCTGCTTTCTCACCTGAGGC |
| SD | ATCGAAGACAGAAAGCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
