13063 Nbl1 Status: ES cell colonies screened / QC no positives
| Gene | Nbl1 |
| Chromosome | 4 |
| Deletion Start | 139,085,617 |
| Deletion End | 139,083,244 |
| Deletion Size | 2,374 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,085,579 | 139,085,598 | CCGTCCTCCCTGTCATGTTG |
| TUP | 139,085,547 | 139,085,570 | CCCGCCACCTATCAACAAGCTGGC |
| TUR | 139,085,516 | 139,085,534 | TTCACACCAGGCACTCTTG |
| TDF | 139,083,486 | 139,083,504 | CAGTCACGAGGGCCTGAAC |
| TDP | 139,083,460 | 139,083,484 | TCTATGTGCAGGGTGAAGACAGCCC |
| TDR | 139,083,426 | 139,083,445 | ATGGGCATGGGAGTGTGGTC |
PCR Primers
| Primer | Sequence |
| SU | CTGAGGACCGTTCAGTCTCC |
| SD | TCCCACATAGGTCCTTCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
