13066 Col4a3 Status: ES cell colonies screened / QC no positives
| Gene | Col4a3 |
| Chromosome | 1 |
| Deletion Start | 82,634,857 |
| Deletion End | 82,664,787 |
| Deletion Size | 29,931 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 82,641,509 | 82,641,526 | TTCTCGCCGCCTCTTCAC |
| TUP | 82,641,530 | 82,641,552 | TGCCTGAGTCGGTCACACTTCGC |
| TUR | 82,641,633 | 82,641,652 | TAGCCGGAGGAGCTTTCAAC |
| TDF | 82,671,264 | 82,671,285 | CTGCACTGCTCTGTTCAATAGC |
| TDP | 82,671,290 | 82,671,319 | AATAAATTGCTTTGCAGGAGGACGAGGTGT |
| TDR | 82,671,327 | 82,671,346 | GACCCTGGGTTTCCCTTCAG |
PCR Primers
| Primer | Sequence |
| SU | ACCAACTTCACCTTTCCCAC |
| SD | GCATGAAAACATCTTCACTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 144 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
