| Gene | Il6ra |
| Chromosome | 3 |
| Deletion Start | 89,890,468 |
| Deletion End | 89,871,113 |
| Deletion Size | 19,356 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,889,783 | 89,889,807 | ACGTTCTTCCTTATGTCCCGTGTAG |
| TUP | 89,889,757 | 89,889,782 | TCCTTCCAGTGTCCGTTTGTCCAGTG |
| TUR | 89,889,734 | 89,889,752 | CCTGTGGCCTCTCCTGATG |
| TDF | 89,871,707 | 89,871,730 | CCTGATAATGTGAGCCCAACAGAC |
| TDP | 89,871,683 | 89,871,704 | CCTCTGGCCCTGGGCAGATTGA |
| TDR | 89,871,656 | 89,871,678 | GCACTTCAAAAGTCACCTGACAC |
PCR Primers
| Primer | Sequence |
| SU | TTTCTATCAAGGGAGCTCGG |
| SD | TCGGAAAGAGAATGGCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13070A-A3 | VGB6 | C57BL/6NTac | |
| 13070A-B9 | VGB6 | C57BL/6NTac | |
| 13070A-C12 | VGB6 | C57BL/6NTac | |
| 13070A-C8 | VGB6 | C57BL/6NTac | |
| 13070A-D12 | VGB6 | C57BL/6NTac | |
