13071 Atp2b2 Status: Cancelled
| Gene | Atp2b2 |
| Chromosome | 6 |
| Deletion Start | 113,842,327 |
| Deletion End | 113,813,792 |
| Deletion Size | 28,536 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,841,537 | 113,841,556 | GCACAGGGACAGGGAAATGG |
| TUP | 113,841,502 | 113,841,529 | AGCCAGCTATAGGCTATGATGGGCAAGA |
| TUR | 113,841,473 | 113,841,495 | CAGGAAAGAACCTGCAAGGACAC |
| TDF | 113,814,366 | 113,814,387 | CAGGGAGGGTATGGGTCAGATG |
| TDP | 113,814,339 | 113,814,364 | TGTCTTTGAAGTCATGCTGCCTGGGA |
| TDR | 113,814,309 | 113,814,329 | AAGAAGGGCAGGTGGAAAGTG |
PCR Primers
| Primer | Sequence |
| SU | GAGCTGTCCCGACCATGTAG |
| SD | CGGAAAGATCTATCTGAAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
