13072 Chrna6 Status: Cancelled
| Gene | Chrna6 |
| Chromosome | 8 |
| Deletion Start | 27,413,699 |
| Deletion End | 27,404,295 |
| Deletion Size | 9,405 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,413,248 | 27,413,272 | TGTCTACTAAGAGTGAGCTTTCCTG |
| TUP | 27,413,220 | 27,413,243 | AAAAGTTGGCTCAGACCTGGCTGC |
| TUR | 27,413,155 | 27,413,174 | GTGTGCATGTGTGCCTGTTC |
| TDF | 27,404,935 | 27,404,955 | GCATCCCTTAGCAGCCTAGAG |
| TDP | 27,404,905 | 27,404,932 | TGCTTAGTCCTATGCAGGAGATCCTCCA |
| TDR | 27,404,875 | 27,404,896 | AGAGGATAGGCCAGAGGATGTG |
PCR Primers
| Primer | Sequence |
| SU | TCCCTGCACAGAGGTCTAGG |
| SD | TGTGTTGCAAGTGTCAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
