13072 Chrna6 Status: Targeting vector QC completed
| Gene | Chrna6 |
| Chromosome | 8 |
| Deletion Start | 28,524,270 |
| Deletion End | 28,514,866 |
| Deletion Size | 9,405 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,523,720 | 28,523,744 | TGTCTACTAAGAGTGAGCTTTCCTG |
| TUP | 28,523,692 | 28,523,715 | AAAAGTTGGCTCAGACCTGGCTGC |
| TUR | 28,523,627 | 28,523,646 | GTGTGCATGTGTGCCTGTTC |
| TDF | 28,515,407 | 28,515,427 | GCATCCCTTAGCAGCCTAGAG |
| TDP | 28,515,377 | 28,515,404 | TGCTTAGTCCTATGCAGGAGATCCTCCA |
| TDR | 28,515,347 | 28,515,368 | AGAGGATAGGCCAGAGGATGTG |
PCR Primers
| Primer | Sequence |
| SU | TCCCTGCACAGAGGTCTAGG |
| SD | TGTGTTGCAAGTGTCAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
