13089 Npy6r Status: Parental BAC obtained
| Gene | Npy6r |
| Chromosome | 18 |
| Deletion Start | 44,435,171 |
| Deletion End | 44,436,337 |
| Deletion Size | 1,167 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,435,467 | 44,435,487 | CCACTGGGTATTTGGGAACAC |
| TUP | 44,435,492 | 44,435,521 | TGTAAACTCACTTCCTACGTGCAAAGTGTC |
| TUR | 44,435,531 | 44,435,555 | TCAACACAAGGGAGAATATGGACAC |
| TDF | 44,435,949 | 44,435,973 | TGATGTTGATTTCCATCGTAGTGAC |
| TDP | 44,435,980 | 44,436,002 | AGCCTGCTGGTTGCCCTTGAACA |
| TDR | 44,436,032 | 44,436,051 | TGGCAGCTCATCAGCATCTC |
PCR Primers
| Primer | Sequence |
| SU | CAAGAGCATGCTGACGTCAT |
| SD | TTCTTTGATGCAAAGGAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
