13091   Npepps     Status: ES cell colonies screened / QC no positives

GeneNpepps
Chromosome11
Deletion Start97,280,455
Deletion End97,248,206
Deletion Size32,250
Genome Build38
MGI IDMGI:1101358
NCBI Gene ID19155
BAC ID380H5
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,279,57597,279,594GACCCTAGCTCCTCTTCTCC
TUP97,279,55397,279,574TATCCGCCTTGCTCTCGCAACC
TUR97,279,52097,279,542CGCCACTGAAGCAATAAATCCAC
TDF97,248,27497,248,298TGTTGGAGAGCTGAATGACAAAATG
TDP97,248,23797,248,262TAGAAGCAGATACACCACCCCTGCCG
TDR97,248,20697,248,227AACTGTGTGACAGCAGCATAGC

PCR Primers

PrimerSequence
SUTGACTTGACCGCTGCGTAG
SDCTAAACAATTACCCACTCATC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR75 bp++-
TDFTDR93 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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