| Gene | Mr1 |
| Chromosome | 1 |
| Deletion Start | 155,146,683 |
| Deletion End | 155,129,265 |
| Deletion Size | 17,419 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,145,796 | 155,145,819 | GTTCACAGGAGAATTGCAGAACTC |
| TUP | 155,145,774 | 155,145,795 | CGATCAGGCCACCCAAGCTGCT |
| TUR | 155,145,750 | 155,145,773 | TCCTTCCTGGCATGAACATTAGTC |
| TDF | 155,129,616 | 155,129,636 | AGTTACTCGTCGGAACCAGTC |
| TDP | 155,129,583 | 155,129,611 | TGCTAGAAAATCAACCCAGCTAGTGGGTA |
| TDR | 155,129,492 | 155,129,514 | GAGGAGAAGAAGCAGGAACTTTC |
PCR Primers
| Primer | Sequence |
| SU | GCAAGCTGAGTCTGAAACCC |
| SD | CTCCTGCTTAGGTGAGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 145 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13093A-A6 | VGB6 | C57BL/6NTac | |
| 13093A-B6 | VGB6 | C57BL/6NTac | |
| 13093A-C5 | VGB6 | C57BL/6NTac | |
| 13093A-E12 | VGB6 | C57BL/6NTac | |
| 13093A-H10 | VGB6 | C57BL/6NTac | |
| 13093A-G8 | VGB6 | C57BL/6NTac | |
