13095 Htr6 Status: ES cell colonies screened / QC no positives
| Gene | Htr6 |
| Chromosome | 4 |
| Deletion Start | 139,074,758 |
| Deletion End | 139,061,289 |
| Deletion Size | 13,470 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,074,573 | 139,074,591 | GCGCAACACGTCTAACTTC |
| TUP | 139,074,549 | 139,074,571 | TCCTGGTGTCGCTCTTCACGTCG |
| TUR | 139,074,523 | 139,074,545 | ATCACCACCAATCCCACCATCAG |
| TDF | 139,062,168 | 139,062,188 | GCGTCTGACCACCAAGCATAG |
| TDP | 139,062,137 | 139,062,159 | CCTTGAAGGCCAGCCTGACCCTC |
| TDR | 139,062,099 | 139,062,119 | GGCAGCCAGGTGACAAAGAAC |
PCR Primers
| Primer | Sequence |
| SU | TGGCTCTATTCCATTCCAGG |
| SD | ATGTCGATTGAGCCCATCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
