13105 Cx3cr1 Status: Cancelled
| Gene | Cx3cr1 |
| Chromosome | 9 |
| Deletion Start | 120,052,331 |
| Deletion End | 120,051,182 |
| Deletion Size | 1,150 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,052,253 | 120,052,274 | GCCTGTTATTTGGGCGACATTG |
| TUP | 120,052,227 | 120,052,252 | TGGCCTTTGGAACCATCTTCCTGTCC |
| TUR | 120,052,191 | 120,052,211 | CACCAGACCGAACGTGAAGAC |
| TDF | 120,051,372 | 120,051,390 | CCTGTGCGGTCATCCTGTC |
| TDP | 120,051,349 | 120,051,371 | CACACCGGCTTCTCGCCAGAGTC |
| TDR | 120,051,295 | 120,051,316 | TCGCTTGTGTAGTGAGTGAAAC |
PCR Primers
| Primer | Sequence |
| SU | AATCTAGTTCCAATTGTTCACC |
| SD | AATGGACACCCGACACACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
