13114 Synj2bp Status: ES cell colonies screened / QC no positives
| Gene | Synj2bp |
| Chromosome | 12 |
| Deletion Start | 81,532,776 |
| Deletion End | 81,502,020 |
| Deletion Size | 30,757 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,532,603 | 81,532,622 | CGGCTTCCCAGAACGCTATC |
| TUP | 81,532,574 | 81,532,599 | CACGGAGCAAAGAGTCCATTGGCATC |
| TUR | 81,532,540 | 81,532,561 | CAGAGTCCTTGGGTCCGTTATC |
| TDF | 81,502,675 | 81,502,693 | TGTGGGCATGGAGGACAAG |
| TDP | 81,502,649 | 81,502,673 | TCCCATCTTAGGAGCAAGAGGCTGA |
| TDR | 81,502,593 | 81,502,615 | GCAGATCCAAACTCTGTCAACAC |
PCR Primers
| Primer | Sequence |
| SU | TCCCACCCAAGTATGCAATC |
| SD | CACAGCATGCCAGCTAAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
