13117 Aplnr Status: Germline transmission achieved
| Gene | Aplnr |
| Chromosome | 2 |
| Deletion Start | 84,976,793 |
| Deletion End | 84,977,961 |
| Deletion Size | 1,169 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,976,903 | 84,976,922 | TCTAGGCACCACAGGCAATG |
| TUP | 84,976,924 | 84,976,947 | CCTGGTGCTCTGGACCGTGTTTCG |
| TUR | 84,976,976 | 84,976,997 | CCAGGCTGGCAATGAAGATGTC |
| TDF | 84,977,492 | 84,977,511 | ATTGAGGGCCTGCGGAAGAG |
| TDP | 84,977,513 | 84,977,535 | CGCCGGCTGCTCAGCATTATCGT |
| TDR | 84,977,543 | 84,977,564 | AGCACAGGGCAAAGGTCACTAC |
PCR Primers
| Primer | Sequence |
| SU | TTGTCAGCCCAGCACTGTAG |
| SD | GGGAAGGGACCCACTAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13117A-A3 | VGB6 | C57BL/6NTac | |
| 13117A-A8 | VGB6 | C57BL/6NTac | |
| 13117A-C6 | VGB6 | C57BL/6NTac | |
| 13117A-D3 | VGB6 | C57BL/6NTac | |
| 13117A-G2 | VGB6 | C57BL/6NTac | |
| 13117A-D2 | VGB6 | C57BL/6NTac | |
