13120 Sspn Status: Cancelled
| Gene | Sspn |
| Chromosome | 6 |
| Deletion Start | 145,934,177 |
| Deletion End | 145,961,513 |
| Deletion Size | 27,337 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 145,934,328 | 145,934,349 | CCTCCCTGCTAGTCAGAGATAC |
| TUP | 145,934,350 | 145,934,374 | TCCGTTCTGGGCTGGGATCATTGTA |
| TUR | 145,934,385 | 145,934,404 | CTGGTGCTTAGGCCGAACAG |
| TDF | 145,961,284 | 145,961,304 | GGGCCTTTGTTTACAGGGATG |
| TDP | 145,961,308 | 145,961,331 | CTGACTGTACCAGCGTCACTGGCA |
| TDR | 145,961,364 | 145,961,384 | GAGGCCACAGACCAGGTTTAG |
PCR Primers
| Primer | Sequence |
| SU | TTATTTAGTGCAGCCGAGCC |
| SD | TGGCTCTAGGTCATCTCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
