13123 Vamp5 Status: ES cell colonies screened / QC positives
| Gene | Vamp5 |
| Chromosome | 6 |
| Deletion Start | 72,330,374 |
| Deletion End | 72,319,246 |
| Deletion Size | 11,129 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,329,455 | 72,329,475 | CGAGAGTACAGGGCAGAGATG |
| TUP | 72,329,425 | 72,329,451 | TTTCAACTGGCTCTCAGCTCTTGAGGA |
| TUR | 72,329,377 | 72,329,398 | TGAACAGCCAGATAACCTAACC |
| TDF | 72,320,064 | 72,320,083 | GGCTTGCCTGCTTATCAGTG |
| TDP | 72,320,037 | 72,320,063 | TGCAACTTCCGGGTTTCCTACAAGTCG |
| TDR | 72,320,015 | 72,320,036 | CCCAAAGGCATCTTGGTGAAAC |
PCR Primers
| Primer | Sequence |
| SU | AGACGCACTCACCAAGGTG |
| SD | TTTACTGAGCTCCAGTGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13123A-E2 | VGB6 | C57BL/6NTac | |
| 13123A-E5 | VGB6 | C57BL/6NTac | |
| 13123A-F3 | VGB6 | C57BL/6NTac | |
| 13123A-G9 | VGB6 | C57BL/6NTac | |
