13129 Raet1d Status: ES cell colonies screened / QC no positives
| Gene | Raet1d |
| Chromosome | 10 |
| Deletion Start | 22,362,718 |
| Deletion End | 22,372,424 |
| Deletion Size | 9,707 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CACAAGCCTTCTGCCATTCAC |
| TUP | | | AAACTCTTTGCAATTGACCTTTC |
| TUR | | | GGAAGCATCTGTTGTGATCTCCTG |
| TDF | | | AACTTCCCCGCTTCCACCTA |
| TDP | | | CCAGCCACTCTACTTC |
| TDR | | | CCTTGGGTATAATATTTCCTCTTCACATT |
PCR Primers
| Primer | Sequence |
| SU | GCACGGGAGCCTAGTACAAC |
| SD | TTGCATATAGCATGGAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
