13133   Ccbp2     Status: ES cell colonies screened / QC positives

GeneCcbp2
Chromosome9
Deletion Start121,817,682
Deletion End121,818,843
Deletion Size1,162
Genome Build37
MGI IDMGI:1891697
NCBI Gene ID59289
BAC ID97E2
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 9

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFCGATGGAGCTTTACCTGCTGAAC
TUPTGGCCGTCTCCAACCTCTTGTTTGTAG
TURCAATGCCAGGCCACAGAGATG
TDFCCGGTCCTCTACGCCTTCTG
TDPCAGTCACCGCTTCCGCCGGTAC
TDRTGCCTGGTGCCATCTCAAC

PCR Primers

PrimerSequence
SUCTCACCTCAGGCATTTAGCC
SDTTGAAGGTCTGAAGGCTAAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTCATTCTCAGTATTGTTTTGCC
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13133A-A5VGB6C57BL/6NTac
13133A-B2VGB6C57BL/6NTac
13133A-B8VGB6C57BL/6NTac
13133A-B11VGB6C57BL/6NTac
13133A-D4VGB6C57BL/6NTac
13133A-D8VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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