13134 Kcnmb4 Status: ES cell colonies screened / QC no positives
| Gene | Kcnmb4 |
| Chromosome | 10 |
| Deletion Start | 116,473,518 |
| Deletion End | 116,446,333 |
| Deletion Size | 27,186 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,473,498 | 116,473,518 | GCGAAGCTCAGGGTGTCTTAC |
| TUP | 116,473,470 | 116,473,494 | TACACGGAAGCCGAAGACAAGAGCA |
| TUR | 116,473,405 | 116,473,426 | CCAGCAGAAGCCGAAGATGAAG |
| TDF | 116,446,602 | 116,446,619 | TTGTGGGCACTGGGATGG |
| TDP | 116,446,574 | 116,446,599 | ATCCCATGCTAACCCTGACTGTGGCC |
| TDR | 116,446,535 | 116,446,558 | GCTTGATTATAGTTCGTGGCTTGG |
PCR Primers
| Primer | Sequence |
| SU | AGGATTCCGAGAGCAGCTC |
| SD | TCCATGTGTTCTGTATATGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
