| Gene | Ssr2 |
| Chromosome | 3 |
| Deletion Start | 88,579,883 |
| Deletion End | 88,588,006 |
| Deletion Size | 8,124 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,579,983 | 88,580,001 | TGGAAGGGAGGGACCTGAC |
| TUP | 88,580,002 | 88,580,029 | CCTGCAGTATAACATCTACAACGTCGGC |
| TUR | 88,580,046 | 88,580,064 | GAAGGAAGCTGGCGAACTC |
| TDF | 88,587,749 | 88,587,767 | GGGAGAGGCTGGCAAAGTC |
| TDP | 88,587,769 | 88,587,790 | TGCTTACATCCTGGGCCCTGCT |
| TDR | 88,587,795 | 88,587,816 | GGGAATGGTCTGGAAGAGAAGG |
PCR Primers
| Primer | Sequence |
| SU | TTTCCGGTCAGATCCGTTTC |
| SD | ACACATCGGTATGGCATTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13135A-A1 | VGB6 | C57BL/6NTac | |
| 13135A-D6 | VGB6 | C57BL/6NTac | |
| 13135A-D12 | VGB6 | C57BL/6NTac | |
