13144 Arl8b Status: ES cell colonies screened / QC no positives
| Gene | Arl8b |
| Chromosome | 6 |
| Deletion Start | 108,783,258 |
| Deletion End | 108,821,630 |
| Deletion Size | 38,373 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,783,690 | 108,783,709 | CGCCTCTCCTAAGTCTCCAC |
| TUP | 108,783,718 | 108,783,743 | ATGGAGTCGCATTGTGAAAGCAGGCA |
| TUR | 108,783,748 | 108,783,766 | TTCACAGAAGCGCCCACAC |
| TDF | 108,821,357 | 108,821,378 | AACCCAGTTCACCGTCGATAAC |
| TDP | 108,821,379 | 108,821,399 | TGCCCTCTTGAAGCTCAGGCA |
| TDR | 108,821,408 | 108,821,429 | TGCTAGGTCTCTTCTGCATGTG |
PCR Primers
| Primer | Sequence |
| SU | GTGACAAGTGAATTGGCACC |
| SD | TGCTGCATCAAGTCCATACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
