| Gene | Lpar6 |
| Chromosome | 14 |
| Deletion Start | 73,238,604 |
| Deletion End | 73,239,644 |
| Deletion Size | 1,041 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,238,651 | 73,238,672 | CACTCTGTACGGGTGCATGTTC |
| TUP | 73,238,673 | 73,238,697 | AGCATGGTCTTCGTGCTTGGGCTGA |
| TUR | 73,238,726 | 73,238,747 | TCTCACTTTGAGGGCACAGATG |
| TDF | 73,239,326 | 73,239,347 | TGTGCCCTACAACATCAACCTC |
| TDP | 73,239,361 | 73,239,389 | TCATGAGGACACAGACCTTTGTTAACTGC |
| TDR | 73,239,403 | 73,239,423 | TCGGGTACATGGTCCTCACTG |
PCR Primers
| Primer | Sequence |
| SU | GGAAGAAGTACCCGAGGACG |
| SD | TTCCGTAAACAACATCTCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13145A-C1 | VGB6 | C57BL/6NTac | |
| 13145A-E6 | VGB6 | C57BL/6NTac | |
