| Gene | Rer1 |
| Chromosome | 4 |
| Deletion Start | 155,082,744 |
| Deletion End | 155,074,925 |
| Deletion Size | 7,820 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,082,273 | 155,082,297 | CCTTGATCTTCTCTTTCCACTCCTC |
| TUP | 155,082,246 | 155,082,270 | TTTCCTCAACCTGACACCCGCATTC |
| TUR | 155,082,198 | 155,082,218 | GCAACACCCAACAAACACCTG |
| TDF | 155,075,280 | 155,075,298 | AGCGCGGGTAAGGCACATC |
| TDP | 155,075,255 | 155,075,279 | CCCACACTGGTGGTGTAGCTGTACC |
| TDR | 155,075,209 | 155,075,233 | AGCCCAACAGAACCTCTGAACAAAG |
PCR Primers
| Primer | Sequence |
| SU | CAGCATTGGAAATGGCTAGG |
| SD | GTGAAATCACCCTCAAAGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13150A-A1 | VGB6 | C57BL/6NTac | |
| 13150A-B12 | VGB6 | C57BL/6NTac | |
| 13150A-C1 | VGB6 | C57BL/6NTac | |
| 13150A-D7 | VGB6 | C57BL/6NTac | |
| 13150A-E4 | VGB6 | C57BL/6NTac | |
| 13150A-G6 | VGB6 | C57BL/6NTac | |
